Structural Proteomics

RNDr. Rostislav Škrabana, PhD

MN423 with peptide Rostislav Skrabana

Structure means mode of existence, which determines the function. Laboratory of Structural Proteomics studies structural and biophysical transformation of proteins involved in neurodegenerative processes and structural principles of antibody effects on disease-modified proteins. Our focus is aimed at proteins with a high degree of structural disorder, so called intrinsically disordered proteins (IDPs), which are often involved in devastating human illnesses, namely tau protein (Alzheimer’s disease), synuclein (Parkinson disease) and prion protein (spongioform encephalopathy).

We employ structural biology tools (X-ray crystallography) corroborated with dynamic biophysical methods of protein structure analysis (surface plasmon resonance, light scattering, molecular spectroscopy, mass spectrometry) and supported by molecular modeling in silico.

We analyse recombinant forms of tau proteins and their interactions with structure-specific monoclonal antibodies.

Lab Members

  • Ing. Ondrej Cehlár, PhD
  • MSc. Branislav Kováčech, PhD
  • MSc. Barbora Majorošová (PhD student)
  • MSc. Andrea Legéňová (technician)

Projects – funding

Biophysics and structure of intrinsically disordered polypeptides studied on the N-terminal tail of tau protein: implications for neurodegenerative tauopathies.
VEGA 02/0163/13
Duration: 01/2013-12/2016
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Structural study of multiple homologous epitopes present on the molecule of intrinsically disordered protein tau.
VEGA 02/0177/15
Duration: 01/2015-12/2018
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Non-globular proteins – from sequence to structure, function and application in molecular physiopathology (NGP-NET)
COST Action BM1405
Duration: 05/2015-03/2019

Alumni

Mgr. Lenka Lovasova, Mgr. Nina Zemanova

Free positions

1 PhD student

Publications