Laboratory of Biomedical Microbiology and Immunology

Mangesh Bhide, DVM, PhD

LBMI

The history
The Laboratory of Biomedical Microbiology and Immunology (LBMI) was established in 1996, the 15th of March. Two organizations, University of veterinary medicine and pharmacy in Košice and Institute of Neuroimmunology, Slovak academy of science, came together with a common goal – “establishment of a sophisticated research unit in the field of host-neuropathogen interactions.

The present
Since its foundation the LBMI is assuring the excellence in research and education. The research activities of the LBMI concern the exploration of host-pathogen interactions. This agenda covers a broad range of highly specialized and specific research activities like: a) effect of mutations in the genes of innate immunity on the susceptibility of the host to pathogens b) exploration of host specificity of pathogen, c) exploration of immune evasive mechanisms of pathogens and d) understanding the underlying molecular principles of ligand:receptor interactions that take place during the evasion of neuropathogen into the CNS. To achieve the goals state of the art techniques used in the LBMI are: different nucleic acid isolation techniques including isolation of genomic DNA, RNA as well as plasmid; nucleic acid amplification (NAAs) like end point PCR, real time PCR, multiplex assays, mutation detection techniques like SSCP, DGGE, high resolution melting (HRM) assays; direct DNA sequencing, gene expression assays like qRT-PCRs, transfection techniques, in-vitro recombinant protein design and production, protein-protein interaction assays like M2H, phage display, affinity ligand binding, pull-down assays and cell culturing techniques.

The main research topics of LBMI:
  • interactions between Borrelia and blood-brain barrier
  • proteomics aspects of Francisella:blood-brain barrier interactions
  • human complement system interacting with Borrelia and Francisella
  • revealing of new proteins involving in Trypanosoma:BMEC interactions
  • development of protein arrays for vector-borne diseases
  • development of domain-specific antibodies against Borrelia, Francisella, Neisseria and Streptococci
  • interaction between various pathogens and astrocytes/microglia

Publications

  1. Pulzova L, Flachbartova Z, Bencurova E, Potocnakova L, Comor L, Schreterova E, Bhide M. Identification of B-cell epitopes of Borrelia burgdorferi outer surface protein C by screening a phage-displayed gene fragment library. Microbiol Immunol. 2016 Sep 13. doi: 10.1111/1348-0421.12438
  2. Csank T, Bhide K, Bencúrová E, Dolinská S, Drzewnioková P, Major P, Korytár Ľ, Bocková E, Bhide M, Pistl J. Detection of West Nile virus and tick-borne encephalitis virus in birds in Slovakia, using a universal primer set. Archives of virology 2016;161(6):1679-1683.
  3. Fecková B, Kimáková P, Ilkovičová L, Szentpéteriová E, Debeljak N, Solárová Z, Sačková V, Šemeláková M, Bhide M, Solár P. Far-western blotting as a solution to the non-specificity of the anti-erythropoietin receptor antibody. Oncology Letters 2016;12(2):1575-1580.
  4. Flachbartova Z, Pulzova L, Bencurova E, Potocnakova L, Comor L, Bednarikova Z, Bhide M. Inhibition of multidrug resistant Listeria monocytogenes by peptides isolated from combinatorial phage display libraries. Microbiological Research 2016;188:34-41.
  5. Kuleš J, Horvatić A, Guillemin N, Galan A, Mrljak V, Bhide M. New approaches and omics tools for mining of vaccine candidates against vector-borne diseases. Molecular BioSystems 2016;12(9):2680-2694
  6. Galan A, Comor L, Horvatić A, Guillemin N, Kuleš J, Mrljak V, Bhide M. Library-based display technologies: where do we stand? Molecular BioSystems 2016.
  7. Guillemin N, Horvatić A, Kuleš J, Galan A, Mrljak V, Bhide M. Omics approaches to probe markers of disease resistance in animal sciences. Molecular BioSystems 2016.
  8. Horvatić A, Kuleš J, Guillemin N, Galan A, Mrljak V, Bhide M. High-throughput proteomics and the fight against pathogens. Molecular BioSystems 2016.
  9. Kreizinger Z, Bhide M, Bencurova E, Dolinska S, Gyuranecz M. Complement sensitivity and factor H binding of European Francisella tularensis ssp. holarctica strains in selected animal species. Acta Veterinaria Hungarica 2015;63(3):275-284.
  10. Csank T, Bhide K, Bencúrová E, Dolinská S, Drzewnioková P, Major P, Korytár Ľ, Bocková E, Bhide M, Pistl J. Detection of West Nile virus and tick-borne encephalitis virus in birds in Slovakia, using a universal primer set. Archives of virology 2016;161(6):1679-1683.
  11. Almeida A, Bassols A, Bendixen E, Bhide M, Ceciliani F, Cristobal S, Eckersall P, Hollung K, Lisacek F, Mazzucchelli G. Animal board invited review: advances in proteomics for animal and food sciences. animal 2015;9(01):1-17.
  12. Bencurova E, Pulzova L, Flachbartova Z, Bhide M. A rapid and simple pipeline for synthesis of mRNA–ribosome–VHH complexes used in single-domain antibody ribosome display. Molecular BioSystems 2015a;11(6):1515-1524.
  13. Bencurova E, Kovac A, Pulzova L, Gyuranecz M, Mlynarcik P, Mucha R, Vlachakis D, Kossida S, Flachbartova Z, Bhide M. Deciphering the protein interaction in adhesion of Francisella tularensis subsp. holarctica to the endothelial cells. Microbial pathogenesis 2015b;81:6-15.
  14. Ferreira AM, Marques AT, Bhide M, Cubric-Curik V, Hollung K, Knight CH, Raundrup K, Lippolis J, Palmer M, Sales-Baptista E. Sequence Analysis of Bitter Taste Receptor Gene Repertoires in Different Ruminant Species. PloS one 2015;10(6):e0124933.
  15. Flachbartova Z, Pulzova L, Bencurova E, Potocnakova L, Comor L, Bednarikova Z, Bhide M. Inhibition of multidrug resistant Listeria monocytogenes by peptides isolated from combinatorial phage display libraries. Microbiological Research 2016;188:34-41.
  16. Madar M, Bencurova E, Mlynarcik P, Almeida AM, Soares R, Bhide K, Pulzova L, Kovac A, Coelho AV, Bhide M. Exploitation of complement regulatory proteins by Borrelia and Francisella. Molecular BioSystems 2015;11(6):1684-1695.
  17. Mlynarcik P, Pulzova L, Bencurova E, Kovac A, Dominguez MA, Hresko S, Bhide MR. Deciphering the interface between a CD40 receptor and borrelial ligand OspA. Microbiological research 2015;170:51-60.
  18. Vlachakis D, Bencurova E, Bhide M, Kossida S. Integrating bioinformatics tools to investigate protein phosphorylation: PeerJ PrePrints; 2015.
  19. Vlachakis D, Bencurova E, Papageorgiou L, Bhide M, Kossida S. Protein phosphorylation prediction: limitations, merits and pitfalls. Journal of Molecular Biochemistry 2015;4(2).
  20. Bhide M., Natarajan S, Hresko S, Aguilar C, Bencurova E (2014) Rapid in vitro protein synthesis pipeline: a promising tool for cost-effective protein array design, Molecular BioSystems 10 (6), 1236-1245.
  21. Pulzova L, M. Bhide (2014) Outer surface proteins of Borrelia: peerless immune evasion tools. Current protein and peptide science. 15 (1), 75-88.
  22. Kreizinger Z, Hornok S, Dán Á, Hresko S, Makrai L, Magyar T, M. Bhide, Erdélyi K, Hofmann-Lehmann R, Gyuranecz M (2013) Prevalence of Francisella tularensis and Francisella-Like Endosymbionts in the Tick Population of Hungary and the Genetic Variability of Francisella-Like Agents. Vector-Borne and Zoonotic Diseases 13: 160-163.
  23. Ward TA, Dudášová Z, Sarkar S, M. Bhide, Vlasáková D, Chovanec M, McHugh PJ (2012) Components of a Fanconi-like pathway control Pso2-independent DNA interstrand crosslink repair in yeast. PLoS genetics 8: e1002884. Cited 10 times, Impact factor – 8.517
  24. Pulzova L, Bencurova E, Mucha R, Mlynarcik P, Csank T, M. Bhide (2012) A possible role of ICAM-1 in OspA mediated borrelial adhesion to BMEC surface. International Journal of Infectious Diseases 16: 237.
  25. Mlynarcik P, Bencurova E, Madar M, Mucha R, Pulzova L, Hresko S, M. Bhide (2012) Development of simple and rapid elution methods for proteins from various affinity beads for their direct MALDI-TOF downstream application. Journal of proteomics 75: 4529-4535.
  26. Bhide M., Bhide K, Pulzova L, Madar M, Mlynarcik P, Bencurova E, Hresko S, Mucha R (2012) Variable regions in the sushi domains 6–7 and 19–20 of factor H in animals and human lead to change in the affinity to factor H binding protein of Borrelia. Journal of proteomics 75: 4520-4528.
  27. Gyuranecz M, Reiczigel J, Krisztalovics K, Monse L, Szabóné GK, Szilágyi A, Szépe B, Makrai L, Magyar T, M. Bhide (2012) Factors Influencing Emergence of Tularemia, Hungary, 1984–2010. Emerging Infectious Diseases 18: 1379. Cited 1 times, Impact factor – 5.993
  28. Bencurova E, Mlynarcik P, M. Bhide (2011) An insight into the ligand–receptor interactions involved in the translocation of pathogens across blood–brain barrier. FEMS Immunology & Medical Microbiology 63: 297-318.
  29. Pulzova L, Kovac A, Mucha R, Mlynarcik P, Bencurova E, Madar M, Novak M, Bhide M (2011) OspA-CD40 dyad: ligand-receptor interaction in the translocation of neuroinvasive Borrelia across the blood-brain barrier. Scientific reports 1.
  30. Kisova-Vargova, Lucia; Mucha, Rastislav; Cernanska, Dana; Bhide Mangesh (2011). Host-dependent differential expression of factor H binding proteins, their affinity to factor H and complement evasion by Lyme and relapsing fever borreliae. Veterinary microbiology: 148:2-4: 341-347. Cited 1 times, Impact factor – 3.327
  31. Pilipčincová I, Bhide M, Dudriková E, Trávniček M (2010) Genotypic characterization of coagulase-negative staphylococci isolated from sheep milk in Slovakia. Acta Veterinaria Brno 79: 269-275. Cited 2 times, Impact factor – 0.534
  32. Mikula I, Bhide M, Pastorekova S (2010) Characterization of ovine TLR7 and TLR8 protein coding regions, detection of mutations and Maedi Visna virus infection. Veterinary immunology and immunopathology 138: 51-59. Cited 10 times, Impact factor – 2.176
  33. Bhide M, Escudero R, Camafeita E, Gil H, Jado I, Anda P (2009) Complement factor H binding by different Lyme disease and relapsing fever Borrelia in animals and human. BMC research notes 2: 134. Cited 12 times, Impact factor – 2.155
  34. Bhide M, Mucha R, Mikula I, Kisova L, Skrabana R, Novak M (2009) Novel mutations in TLR genes cause hyporesponsiveness to Mycobacterium avium subsp. paratuberculosis infection. BMC genetics 10: 21. Cited 17 times, Impact factor – 2.23
  35. Mucha R, Bhide M, Chakurkar E, Novak M, Mikula I (2009) Toll-like receptors TLR1, TLR2 and TLR4 gene mutations and natural resistance to Mycobacterium avium subsp. paratuberculosis infection in cattle. Veterinary immunology and immunopathology 128: 381-388. Cited 40 times, Impact factor – 1.963
  36. Pulzova L, Bhide M, Andrej K (2009) Pathogen translocation across the blood–brain barrier. FEMS Immunology & Medical Microbiology 57: 203-213. Cited 12 times, Impact factor – 2.335
  37. Bugos O; Bhide, M; Zilka, (2009) Beyond the Rat Models of Human Neurodegenerative Disorders Cellular and Molecular Neurobiology. 29:6-7: 859-869. Cited 10 times, Impact factor – 2.107
  38. Bhide M, Yilmaz Z, Golcu E, Torun S, Mikula I (2008) Seroprevalence of anti-Borrelia burgdorferi antibodies in dogs and horses in Turkey. Annals of agricultural and environmental medicine: AAEM 15: 85-90. Cited 18 times, Impact factor – 1.443
  39. Escudero R, Toledo A, Gil H, Kovácsová K, Rodríguez-Vargas M, Jado I, García-Amil C, Lobo B, Bhide M, Anda P (2008) Molecular method for discrimination between Francisella tularensis and Francisella-like endosymbionts. Journal of clinical microbiology 46: 3139-3143. Cited 14 times, Impact factor – 3.945
  40. García-Esteban C, Gil H, Rodríguez-Vargas M, Gerrikagoitia X, Barandika J, Escudero R, Jado I, García-Amil C, Barral M, García-Pérez, M Bhide, Pedro Anda (2008) Molecular method for Bartonella species identification in clinical and environmental samples. Journal of clinical microbiology 46: 776-779. Cited 17 times, Impact factor – 3.945.
  41. Bhide M, Chakurkar E, Tkacikova L, Barbuddhe S, Novak M, Mikula I (2006) IS900 PCR-based detection and characterization of Mycobacterium avium subsp. paratuberculosis from buffy coat of cattle and sheep. Veterinary microbiology 112: 33-41. Cited 27 times, Impact factor – 2.370
  42. Dmitriev A, Bhide M, Mikula I (2006) cpn60 gene-based multiplex-PCR assay for simultaneous identification of Streptococcal species. Acta Veterinaria Brno 75: 235-240. Cited 5 times, Impact factor – 0.403
  43. Kováčová E, Sekeyova Z, Trávniček M, Bhide M, mardzinova s, čurlik j, španelová d (2006) Monitoring of humans and animals for the presence of various Rickettsiae and Coxiella burnetii by serological methods. Annals of the New York Academy of Sciences 1078: 587-589. Cited 5 times, Impact factor – 2.303
  44. Świderek W, Bhide M, Gruszczyńska J, Soltis K, Witkowska D, Mikula I (2006) Toll-like receptor gene polymorphism and its relationship with somatic cell concentration and natural bacterial infections of the mammary gland in sheep. Folia microbiologica 51: 647-652. Cited 6 times, Impact factor – 1.172
  45. Tkáčiková Ľ, Bhide M, Imrichová D, Mikula Sr I (2006) The analysis of prion protein gene polymorphism in Slovakian white shorthaired goats. Folia Veterinaria 50: 146-150. Cited 1 times, Impact factor – 0.545
  46. Bhide M, Travnicek M, Levkutova M, Curlik J, Revajova V, Levkut M (2005) Sensitivity of Borrelia genospecies to serum complement from different animals and human: a host–pathogen relationship. FEMS Immunology & Medical Microbiology 43: 165-172. Cited 28 times, Impact factor – 2.243
  47. Tkáčiková Ľ, Bhide M., Mikula I (2005) Asymmetric PCR-SSCP: a useful tool for detection of OLA-DRB1 (MHC Class II) gene polymorphism in Slovak Improved Valachian sheep. Acta Veterinaria Brno 74: 275. Cited 2 times, Impact factor – 0.415
  48. Tkacikova L, Rolková G, Bires J, Novak P, Kontsekova E, Imrichova D, Bhide M, Hanusovska E, Mikula I, Novak M (2005) PrP gene polymorphism in sheep breeds in Slovakia and susceptibility to scrapie. Acta virologica 50: 257-261. Cited 2 times, Impact factor – 0.81
  49. Bhide M., Travnicek M, Curlik A, Stefancikova A (2004) The importance of dogs in eco-epidemiology of Lyme borreliosis: a review. Veterinarni Medicina 49. Cited 9 times, Impact factor – 0.659
  50. Bhide M, Curlik J, Travnicek M, Lazar P (2004) Protein A/G dependent ELISA a promising diagnostic tool in Lyme disease seroprevalence in game animals and hunting dogs. Comparative immunology, microbiology and infectious diseases 27: 191-199. Cited 9 times, Impact factor – 2.921
  51. Marcinak,S; Sokol,J; Bystricky,P; Popelka,P; Turek,P; Bhide, M; Mate, D. (2004) Determination of lipid oxidation level in broiler meat by liquid Chromatography. Journal of AOAC international, 87 (5): 1148-1152. Cited 13 times, Impact factor – 1.322
  52. Stefancikova, A; Bhide, M; Pet’ko, B; et al. (2004) Anti-Borrelia antibodies in rodents: Important hosts in ecology of Lyme disease. Annals of agricultural and environmental medicine. 11: 2: 209-213. Cited 7 times, Impact factor – 2.022
  53. Travnicek M, Stefancikova A, Nadzamova D, Stanko M, Cislakova L, Pet’ko B, Mardzinová S, Bhide M (2003) Immunoglobulin G antibodies to Borrelia burgdorferi in game animals and small mammals in eastern Slovakia. Revue scientifique et technique-Office international des épizooties 22: 1035-1041. Cited 4 times, Impact factor – 1.238
  54. Travnicek M, Stefancikova A, Nadzamova D, Stanko M, Cislakova L, Pet’ko B, Mardzinova S, Bhide M (2002) Seroprevalence of anti-Borrelia burgdorferi antibodies in sheep and goats from mountainous areas of Slovakia. Annals of agricultural and environmental medicine: AAEM 9: 153. Cited 6 times, Impact factor – 2.111
  55. Travnicek M, Cislakova L, Deptuła W, Stosik M, Bhide M (2002) Wild pigeons and pheasants- a source of Chlamydophila psittaci for humans and animals. Annals of agricultural and environmental medicine: AAEM 9: 253. Cited 13 times, Impact factor – 2.111
  56. Travnicek M, Kovacova D, Bhide M, Zubricky P, Cislakova L (2002) Field evaluation of an iELISA and CF test for detection of IgG antibodies against Chlamydophila abortus in goats, sheep and rams. Veterinary Medicina- 47: 195-198. Cited 2 times, Impact factor – 0.635
  57. Travniček M, Kováčová D, Bhide M, Zubrický P, Čisláková L (2003) Detection of IgG antibodies against Chlamydophila abortus in sheep with reproductive disorders. Acta Veterinaria Brno 72: 95. Cited 5 times, Impact factor – 0.514
  58. Bhide M, Paturkar A, Sherikar A, Waskar V (2001) Presensitization of microorganisms by acid treatments to low dose gamma irradiation with special reference to Bacillus cereus. Meat science 58: 253-258. Cited 32 times, Impact factor – 2.223