Finished Projects

View projects over the past five years.

International

BIOMARKAPD – Biomarkery pre Alzheimerovu a Parkinsonovu chorobu
Biomarkers for Alzheimer’s disease and Parkinson’s disease
Program: Multilateral – other
Project leader: Doc. MVDr. Žilka Norbert DrSc.
Annotation: Neurodegenerative disorders, represented mostly by Alzheimer’s disease (AD) and Parkinson’sdisease (PD), are characterised by progressive neuronal impairment and death. In spite of the brain’sknown capacity for regeneration, lost neurons generally cannot be replaced. Therefore, drugs aimedat inhibiting neurodegenerative processes are likely to be most effective if the treatment is initiated asearly as possible in the disease process. However, clinical manifestations in early disease stages areoften difficult to diagnose. This is where biomarkers, specifically reflecting the onset of pathology mayhave a profound impact on diagnosis and detection of treatment effects in the near future. A triplet ofcerebrospinal fluid (CSF) biomarkers for AD, total and hyperphosphorylated tau that reflect AD-typeaxonal degeneration, and the 42 amino acid isoform of amyloid beta that reflects senile plaque pathology, has already been established for early detection of AD before the onset of dementia. With regards to PD, the most promising biomarker is CSF alpha-synuclein. However, large variations in all biomarker measurements have been reported between studies, both between and within centres andlaboratories. Such variations may be caused by pre-analytical, analytical, or assay-related factors andseriously jeopardize the introduction of biomarkers in clinical routine and trials around the world.
Duration: 1.6.2012 – 1.6.2015
NANONET – Nanomechanika sietí intermediálnych filamentov
NANOMECHANICS OF INTERMEDIATE FILAMENT NETWORKS – NANONET
Program: COST
Project leader: Doc. MVDr. Žilka Norbert DrSc.
Duration: 25.5.2010 – 24.5.2014
Patologické štiepenie tau: samoobnovujúci sa propagátor neurofibrilárnej degenerácie v Alzheimerovej chorobe
Tau truncation: the self-renewing propagator of neurofibrillary degeneration in Alzheimer’s disease
Program: UNIDO
Project leader: Mgr. Kováčech Branislav PhD.
Duration: 1.1.2011 – 31.12.2013
JUMPAHEAD – Koordinačná aktivita na podporu implementácia inociatívy Spoločné programovanie v boji proti neurodegenračným ochoreniam, zvlášť Alzheimerovej chorobe.
COORDINATION ACTION IN SUPPORT OF THE IMPLEMENTATION OF A JOINT PROGRAMMING INITIATIVE FOR COMBATING NEURODEGENERATIVE DISEASES, IN PARTICULAR ALZHEIMER‘S DISEASE
Program: FP7
Project leader: prof. MVDr. Novák Michal DrSc., Dr.h.c.
Duration: 1.9.2010 – 31.8.2013
ALCOVE – Spoločná európska evaluácia problematiky Alzheimerovej choroby
ALzheimer COoperative Valuation in Europe
Program: Multilateral – other
Project leader: prof. MVDr. Novák Michal DrSc., Dr.h.c.
Duration: 1.4.2011 – 31.3.2013

National

BIOFYZIKÁLNA ANALÝZA N-KONCOVEJ DOMÉNY PROTEÍNU TAU MODEL PRE PRIRODZENE NEUSPORIADANÝ POLYPEPTID: DÔSLEDKY PRE NEURODEGENERAČNÉ TAUOPÁTIE
Biophysics and structure of intrinsically disordered polypeptides studied on the N-terminal tail of tau protein: implications for neurodegenerative tauopathies
Program: VEGA
Project leader: RNDr. Škrabana Rostislav PhD.
Duration: 1.1.2013 – 31.12.2016
KONVERZIA NEURONÁLNÉHO PROTEÍNU TAU NA PATOLOGICKÉ FORMY VO ZVIERACOM MODELI NEUROFIBRILÁRNEJ DEGENERÁCIE
Conversion of the neuronal protein tau into pathological forms in the animal model of neurofibrillary degeneration
Program: VEGA
Project leader: Mgr. Kováčech Branislav PhD.
Duration: 1.1.2013 – 31.12.2016
MOLEKULÁRNE MECHANIZMY DYSREGULÁCIE PROTEOSTÁZY V NEURODEGENERATÍVNYCH PROTEINOPÁTIÁCH
Identification of molecular mechanisms underlying dysregulated proteostasis in neurodegenerative proteinopathy
Program: VEGA
Project leader: RNDr. Žilková Monika PhD.
Duration: 1.1.2014 – 31.12.2016
ETIOPATOGENÉZA NEURODEGENERATÍVNYCH OCHORENÍ: VÝZNAM POSTTRANSKRIPČNEJ ÚPRAVY RNA PRE VZNIK A PROGRESIE SPORADICKÝCH TAUOPÁTIÍ A ALZHEIMEROVEJ CHOROBY
Etiopathogenesis of neurodegenerative diseases: focus on RNA processing regulation in development and progression of sporadic tauopathies and Alzheimer´s disease
Program: SRDA
Project leader: doc. RNDr. Filipčík Peter CSc.
Duration: 1.10.2013 – 30.9.2016
ŠTÚDIUM FUNKCIE TAU PROTEÍNU V JADRÁCH NEURÓNOV
Studies of tau protein functions in nuclei of neurons
Program: VEGA
Project leader: Mgr. Baráth Peter PhD.
Annotation: Neurofibrillary tangles, one of the hallmarks of Alzheimer\’s disease (AD),are composed of missfolded tau protein that belongs to the family of microtubule-associated proteins. The transition between normal and pathological tau proteins is driven by post-translational modifications and partial proteolytic cleavage. It is generally assumed that the main function of tau involves regulation of the dynamics of neuronal microtubules. Recently, this typical cytosolic protein has been shown to translocate to the nucleus where it might participate in DNA protection upon stress. Within the project, we will study nuclear tau shuttling mechanism using cellular models expressing truncated forms, some of which cause development of AD-like symptoms in transgenic rat. We will also explore the mechanism of DNA protection that includes description of nuclear tau interactome and FISH analysis of chromosomal regions recognized by tau. The study will provide new perspective on physiological and pathological roles of tau protein.
Duration: 1.1.2012 – 31.12.2015
UBIQUITÍN- PROTEAZÓMOVÝ SYSTÉM A JEHO AKTIVITA V BUNKOVOM MODELY NEURODEGRADÁCIE: IMPLIKÁCIE PRE ETIOPATOGENÉZU DEGENERATÍVNYCH OCHORENÍ MOZGU
Ubiquitin proteasomal system and it’s activity in cellular model of neurodegeneration: implications for etiopathogenesis of degenerative diseases of brain
Program: VEGA
Project leader: doc. RNDr. Filipčík Peter CSc.
Duration: 1.1.2013 – 31.12.2015
Brain centrum – CENTRUM EXCELENTNOSTI PRE VÝSKUM MOZGU SAV
Program: Centrá excelentnosti SAV
Project leader: Doc. MVDr. Žilka Norbert DrSc.
Duration: 4.8.2011 – 3.8.2015
MECHANIZMUS INTERAKCIE IMUNITNÉHO A NERVOVÉHO SYSTÉMU V PROCESE NEURODEGENERÁCIE MOZGU
Mechnism of immune and neuronal system interaction in the brain during neurodegeneration
Program: SRDA
Project leader: RNDr. Žilková Monika PhD.
Duration: 1.7.2012 – 30.6.2015
RIZIKOVÉ FAKTORY A PROTEOMICKÝ RUKOPIS KOGNITÍVNYCH DYSFUNKCIÍ ANIMÁLNYCH MODELOV PRE ĽUDSKÉ DEMENCIE
Risk factors and proteomic signature of cognitive dysfunctions in animal models for human dementias
Program: SRDA
Project leader: Doc. MVDr. Žilka Norbert DrSc.
Duration: 1.7.2012 – 30.6.2015
ANALÝZY INERAKČNÝCH PARTNEROV PRIÓNOVÉHO PROTEÍNU A ICH FUNKČNÝ VÝZNAM
Analysis of prion protein interaction partners and their functional significance
Program: VEGA
Project leader: prof. RNDr. Kontseková Eva DrSc.
Duration: 1.1.2012 – 31.12.2014
VPLYV RNA SEKUNDÁRNEJ ŠTRUKTÚRY NA EFEKTIVITU ZOSTRIHU PREKURZOROVEJ MRNA. IMPLIKÁCIA PRE REGULÁCIU SYNTÉZY TAU EXÓN 10 +/- IZOFORIEM
Effect RNA secondary structure on RNA splicing effciency. Implication for regulation sythesis of tau exon10 +/-
Program: VEGA
Project leader: Mgr. Královičová Jana PhD
Duration: 1.1.2012 – 31.12.2014
JE STRES JEDNÝM Z PODSTATNÝCH FAKTOROV NEURODEGENERAČNÉHO PROCESU PRI ALZHEIMEROVEJ CHOROBE?
Is stress a crucial factor in the process of neurodegeneration accompanying Alzheimer´s disease?
Program: SRDA
Project leader: doc. RNDr. Filipčík Peter CSc.
Duration: 1.5.2011 – 31.10.2014
TauDecode – IDENTIFIKÁCIA POST-TRANSLAČNÝCH MODIFIKÁCIÍ NEURONÁLNEHO PROTEÍNU TAU ZODPOVEDNÝCH ZA NEUROFIBRILÁRNU DEGENERÁCIU V TAUOPÁTIÁCH.
Identification of the posttranslational modifications of the neuronal protein tau leading to neurofibrillary degeneration in tauopathies
Program: SRDA
Project leader: Mgr. Kováčech Branislav PhD.
Annotation: Neurofibrillary degeneration consisting of misfolded neuronal protein tau is the key pathological feature of tauopathies, affecting about 13% of people over the age of 65 and half of those over 85 in the developed countries. The extent of the tau pathology directly correlates with the clinical progression of the disease. It is assumed that halting the tau misfolding process in neurofibrillary pathology would lead to successful therapies preventing the cognitive decline. Hyperphosphorylation and truncation of tau are thought to play the principal role in the induction of its pathology. However, the identity and temporal order of the individual phosphorylation events (there are 45 confirmed phospho-sites on tau) that are required for its misfolding have not been revealed, yet. Furthermore, the temporal relationship of truncation and phosphorylation events on tau is also unknown. In this project we will address the question of the identity and temporal order of the individual phosphorylation patterns and truncation events in the misfolding process of normal tau protein into insoluble aggregates. We will use a unique rat model faithfully reproducing all aspects of the complete cascade of human neurofibrillary degeneration. The results will thus provide a clue for the understanding the paradox how can a highly soluble structureless flexible protein turn insoluble and highly structured in the disease.
Duration: 1.5.2011 – 30.4.2014
ANALÝZA BIOMARKEROV V CEREBROSPINÁLNEJ TEKUTINE TRANSGÉNNYCH ANIMÁLNYCH MODELOV PRE ĽUDSKÉ TAUOPATIE.
Analysis of biomarkers in cerebrospinal fluid in transgenic animal models for human tauopathies.
Program: VEGA
Project leader: MUDr. Kosoň Peter PhD.
Duration: 1.1.2011 – 31.12.2013
METABOLOMICKÁ ANALÝZA POTKANIEHO MODELU PRE TAUOPÁTIE.
Metabolomic analysis of rat model of tauopathy.
Program: VEGA
Project leader: prof. MVDr. Novák Michal DrSc., Dr.h.c.
Duration: 1.1.2011 – 31.12.2013
MITOCHONDRIÁLNE OXIDAČNO-REDUKČNÉ SIGNÁLNE DRÁHY V PATOGENÉZE DEGENERÁCIE CENTRÁLNEHO NERVOVÉHO SYSTÉMU.
Mitochondrial redox signaling pathways in the pathogenesis of degeneration of central nervous system.
Program: VEGA
Project leader: RNDr. Čente Martin PhD.
Duration: 1.1.2011 – 31.12.2013
MOLEKULOVÝ MECHANIZMUS BUNKOVEJ SMRTI PRI ALZHEIMEROVEJ CHOROBE.
Molecular mechanism of cell death in Alzheimer’s disease.
Program: VEGA
Project leader: RNDr. Žilková Monika PhD.
Duration: 1.1.2011 – 31.12.2013
PROTEOMICKÁ ANALÝZA ZDRAVÉHO A CHORÉHO TAU PROTEÍNU U ALZHEIMEROVEJ CHOROBY.
Proteomic analysis of physiological and pathological tau protein by Alzheimer´s disease.
Program: VEGA
Project leader: MUDr. Novák Petr PhD.
Duration: 1.1.2011 – 31.12.2013
ŠTÚDIUM RÔZNYCH ÚNIKOVÝCH STRATÉGIÍ NEUROINVAZÍVNYCH KMEŇOV FRANCISELLA A BORRELIA PRED KOMPLEMENTOVÝM SYSTÉMOM.
Study of multiple complement evasion strategies used by neuroinvasive Francisella and Borrelia.
Program: VEGA
Project leader: Prof. MVDr. Mikula Ivan DrSc.
Duration: 1.1.2011 – 31.12.2013
ZÁPALOVÉ DRÁHY OVPLYVNENÉ GENETICKÝM POZADÍM V TAUOPATII.
Inflammatory pathways influenced by the gentic background in tauopathy.
Program: VEGA
Project leader: Doc. MVDr. Žilka Norbert DrSc.
Duration: 1.1.2011 – 31.12.2013
KOMPLEXNÝ VÝSKUM ŠTRUKTÚRY PRIRODZENE NEUSPORIADANÉHO PROTEÍNU TAU VYSTUPUJÚCEHO V NEURODEGENERAČNÝCH OCHORENIACH.
Complex approach to structural study of intrinsically disordered protein tau associated with neurodegenerations.
Program: SRDA
Project leader: RNDr. Škrabana Rostislav PhD.
Duration: 1.9.2009 – 31.8.2013
SKRÍNINGOVÝ SYSTÉM PRE IDENTIFIKÁCIU POTENCIÁLNYCH BIOLOGICKÝCH MARKEROV PRE SKORÚ DIAGNOSTIKU PARKINSONOVEJ CHOROBY
Program: EU Structural Funds Research & Development
Project leader: doc. RNDr. Filipčík Peter CSc.
Duration: 1.11.2010 – 30.4.2013
MOLEKULOVÉ MECHANIZMY NEUROTOXICITY INDUKOVANÉ EXPRESIOU PRIRODZENE NEZVINUTÝCH PROTEÍNOV.
Molecular mechanisms of neurotoxicity induced by expression of naturaly unfolded proteins
Program: VEGA
Project leader: doc. RNDr. Filipčík Peter CSc.
Duration: 1.1.2010 – 31.12.2012
MONOKLONOVÉ PROTILÁTKY AKO NÁSTROJ NA ANALÝZU KONFORMAČNÝCH ZMIEN TAU PROTEÍNU PRI ALZHEIMEROVEJ CHOROBE
Monoclonal antibodies as a tool for study of conformational changes of tau protein in Alzheimer´s disease
Program: VEGA
Project leader: RNDr. Paulenka-Ivanovová Natália PhD.
Duration: 1.1.2010 – 31.12.2012
PRIRODZENE NEZVINUTÝ PROTEÍN TAU A JEHO ŠTRUKTURÁLNE ZMENY ANALYZOVANÉ POMOCOU IMUNOLOGICKÉHO IMPRINTU – KONFORMAČNE-ZÁVISLEJ MONOKLONOVEJ PROTILÁTKY
Intrinsically disordered protein tau and its structural changes analyzed by its immunological imprint – conformation-specific monoclonal antibody
Program: VEGA
Project leader: Mgr. Kováčech Branislav PhD.
Duration: 1.1.2010 – 31.12.2012
ŠTÚDIUM ŠTVRTEJ MIKROTUBULY-VIAŽÚCEJ OBLASTI NEUSPORIADANÉHO PROTEÍNU TAU KOMPLEMENTÁRNYMI METÓDAMI ŠTRUKTÚRNEJ BIOLÓGIE VO VZŤAHU K FYZIOLÓGII A PATOLÓGII TAU
Fourth microtubule-binding region of disordered tau protein studied by complementary methods of structural biology: insight into its role in physiology and pathology
Program: VEGA
Project leader: RNDr. Škrabana Rostislav PhD.
Duration: 1.1.2010 – 31.12.2012
VPLYV PATOLOGICKÝCH FORIEM TAU PROTEÍNU NA ŠTRUKTURÁLNE ZMENY NEUROVASKULÁRNEJ JEDNOTKY U POTKANIEHO MODELU PRE ALZHEIMEROVU CHOROBU
Impact of pathological forms of tau protein on structural changes of neurovascular unit in the rat model of Alzheimer’s disease
Program: VEGA
Project leader: Ing. Bugoš Ondrej PhD
Duration: 1.1.2010 – 31.12.2012
TRANSKRIPČNÁ A PROTEOMICKÁ ANALÝZA OXIDAČNO-REDUKČNÉHO SYSTÉMU V NEURÓNOCH EXPRIMUJÚCICH PATOLOGICKÉ FORMY PROTEÍNU TAU
Transcriptional and proteomic analysis of redox system in neurons expressing pathological tau protein isoforms
Program: SRDA
Project leader: doc. RNDr. Filipčík Peter CSc.
Duration: 1.9.2009 – 31.8.2012